Recombinant Human IFN-gamma Protein (2022)

285-IF 402 Citations 22 Reviews Datasheet / COA / SDS

GMP Version Available: 285-GMP

GMP

2 Images

Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human IFN-gamma Protein Summary

Product Specifications

Purity

>97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.01 EU per 1 μg of the protein by the LAL method.

Activity

Measured in anti-viral assays using HeLa human cervical epithelial carcinoma cells infected with encephalomyocarditis (EMC) virus. Meager, A. (1987) in Lymphokines and Interferons, a Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 129. The ED50 for this effect is 0.15-0.75 ng/mL.
The specific activity of Recombinant Human IFN-gamma is approximately2 x 104 IU/μg, which is calibrated against humanIFN-gamma Standard (NIBSC code: 87/586).Specific activity is for reference purposes only and is not routinely tested.

Source

E. coli-derived human IFN-gamma protein
Gln24-Gln166 with an N-terminal Met

Accession #

N-terminal Sequence
Analysis

Met

Predicted Molecular Mass

16.9kDa

SDS-PAGE

17 kDa, reducing conditions

Product Datasheets

285-IF (with carrier)

Product Datasheet

COA

SDS

285-IF/CF (carrier free)

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

285-IF

Formulation Lyophilized from a 0.2 μm filtered solution in Sodium Succinate, Mannitol and Tween® 80 with BSA as a carrier protein.
Reconstitution Reconstitute at 0.2 mg/mL insterile, deionized water.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

285-IF/CF

Formulation Lyophilized from a 0.2 μm filtered solution in Sodium Succinate, Mannitol and Tween® 80.
Reconstitution Reconstitute at 0.2 mg/mL insterile, deionized water.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
(Video) Learn IFN Gamma in 3 minutes | Interferon gamma, IFNG

Data Images

SDS-PAGE View Larger

1 μg/lane of Recombinant Human IFN-gamma was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 17 kDa.

Recombinant Human IFN-gamma (Catalog # 285‑IF) demonstrates anti-viral activity in HeLa human cervical epithelial carcinoma cells infected with encephalomyocarditis (EMC) virus. The activity is over 2-fold greater than the top competitor's IFN-gamma.

Reconstitution Calculator

Reconstitution Calculator

Background: IFN-gamma

Interferon-gamma (IFN-gamma ), also known as type II or immune interferon, exerts a wide range of immunoregulatory activities and is considered to be the prototype proinflammatory cytokine (1, 2). Mature human IFN-gamma exists as a non-covalently linked homodimer of 20-25 kDa variably glycosylated subunits (3). It shares 90%amino acid (aa) sequence identity with rhesus IFN-gamma, 59%‑64%with bovine, canine, equine, feline, and porcine IFN‑ gamma, and 37%‑43%with cotton rat, mouse, and rat IFN-gamma. IFN-gamma dimers bind to IFN-gamma RI ( alpha subunits) which then interact with IFN-gamma RII ( beta subunits) to form the functional receptor complex of two alpha and two beta subunits. Inclusion of IFN-gamma RII increases the binding affinity for ligand and the efficiency of signal transduction (4, 5). IFN-gamma is produced by a variety of immune cells under inflammatory conditions, notably by T cells and NKcells (6). It plays a key role in host defense by promoting the development and activation of Th1 cells, chemoattraction and activation of monocytes and macrophages, up‑regulation of antigen presentation molecules, and immunoglobulin class switching in Bcells. It also exhibits antiviral, antiproliferative, and apoptotic effects (6, 7). In addition, IFN-gamma functions as an anti-inflammatory mediator by promoting the development of regulatory T cells and inhibiting Th17 cell differentiation (8, 9). The pleiotropic effects of IFN-gamma contribute to the development of multiple aspects of atherosclerosis (7).

References

  1. Billiau, A. and P. Matthys (2009) Cytokine Growth Factor Rev. 20:97.
  2. Pestka, S. et al. (2004) Immunol. Rev. 202:8.
  3. Gray, P.W. and D.V. Goeddel (1982) Nature 298:859.
  4. Marsters, S.A. et al. (1995) Proc. Natl. Acad. Sci. 92:5401.
  5. Krause, C.D. et al. (2000) J. Biol. Chem. 275:22995.
  6. Schroder, K. et al. (2004) J. Leukoc. Biol. 75:163.
  7. McLaren, J.E. and D.P. Ramji (2009) Cytokine Growth Factor Rev. 20:125.
  8. Muhl, H. and J. Pfeilschifter (2003) Int. Immunopharmacol. 3:1247.
  9. Kelchtermans, H. et al. (2008) Trends Immunol. 29:479.

Long Name

Interferon gamma

Entrez Gene IDs

3458 (Human); 15978 (Mouse); 25712 (Rat); 396991 (Porcine); 281237 (Bovine); 403801 (Canine); 493965 (Feline)

Alternate Names

IFG; IFI; IFNG; IFNgamma; IFN-gamma; Immune interferon; interferon gamma; interferon, gamma

Citations for Recombinant Human IFN-gamma Protein

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

402 Citations: Showing 1 - 10
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  1. The Thermal Dose of Photothermal Therapy Generates Differential Immunogenicity in Human Neuroblastoma Cells
    Authors: P Sekhri, DK Ledezma, A Shukla, EE Sweeney, R Fernandes
    Cancers, 2022;14(6):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Cell Culture
  2. Cooperative effects of RIG-I-like receptor signaling and IRF1 on DNA damage-induced cell death
    Authors: DY Zander, SS Burkart, S Wüst, VG Magalhães, M Binder
    Cell Death & Disease, 2022;13(4):364.
    Species: Human
    Sample Types: Whole Cell
    Applications: Bio-assay
  3. MMP-10 from M1 macrophages promotes pulmonary vascular remodeling and pulmonary arterial hypertension
    Authors: PL Chi, CC Cheng, CC Hung, MT Wang, HY Liu, MW Ke, MC Shen, KC Lin, SH Kuo, PP Hsieh, SR Wann, WC Huang
    International journal of biological sciences, 2022;18(1):331-348.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  4. Dendrobium nobile Lindley Administration Attenuates Atopic Dermatitis-like Lesions by Modulating Immune Cells
    Authors: S Hong, EY Kim, SE Lim, JH Kim, Y Sohn, HS Jung
    International Journal of Molecular Sciences, 2022;23(8):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  5. Interferon-gamma promotes monocyte-mediated lung injury during influenza infection
    Authors: T Schmit, K Guo, JK Tripathi, Z Wang, B McGregor, M Klomp, G Ambigapath, R Mathur, J Hur, M Pichichero, J Kolls, MN Khan
    Cell Reports, 2022;38(9):110456.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  6. Inhibiting Type I arginine methyltransferase activity promotes the T cell mediated antitumor immune response
    Authors: A Fedoriw, L Shi, S O'Brien, KN Smitheman, Y Wang, J Hou, C Sherk, S Rajapurkar, J Laraio, LJ Williams, C Xu, G Han, Q Feng, MT Bedford, L Wang, O Barbash, RG Kruger, P Hwu, HP Mohammad, W Peng
    Cancer Immunology Research, 2022;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  7. The inflammatory regulation of TRPA1 expression in human A549 lung epithelial cells
    Authors: S Luostarine, M Hämäläinen, N Hatano, K Muraki, E Moilanen
    Pulmonary pharmacology & therapeutics, 2021;70(0):102059.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  8. Phospholipase A2 enzymes represent a shared pathogenic pathway in psoriasis and pityriasis rubra pilaris
    Authors: S Shao, J Chen, WR Swindell, LC Tsoi, X Xing, F Ma, R Uppala, MK Sarkar, O Plazyo, AC Billi, R Wasikowski, KM Smith, P Honore, VE Scott, E Maverakis, JM Kahlenberg, G Wang, NL Ward, PW Harms, JE Gudjonsson
    JCI Insight, 2021;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  9. FKBP3 Induces Human Immunodeficiency Virus Type 1 Latency by Recruiting Histone Deacetylase 1/2 to the Viral Long Terminal Repeat
    Authors: X Yang, X Zhao, Y Zhu, Y Shen, Y Wang, P Lu, Z Jiang, H Pan, J Yang, J Xun, L Zhao, J Wang, Z Liang, X Shen, Y Liang, Q Lin, H Liang, L Jin, D Zhang, J Liu, B Wang, S Jiang, J Xu, H Wu, H Lu, H Zhu
    MBio, 2021;0(0):e0079521.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  10. A Novel Kinase Inhibitor AX-0085 Inhibits Interferon-gamma-Mediated Induction of PD-L1 Expression and Promotes Immune Reaction to Lung Adenocarcinoma Cells
    Authors: J Kim, H Jang, GJ Lee, Y Hur, J Keum, JK Jo, SE Yun, SJ Park, YJ Park, MJ Choi, KS Kim, J Kim
    Cells, 2021;11(1):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Cell Culture

FAQs

  1. Why does Recombinant Human IFN-gamma protein, Catalog #285-IF,have theamio acid substitution R160Q?

    • The sequence of R&D Systems' rhIFN-gamma, Catalog # 285-IF, is based on the the following publication in Nature:[https://www.ncbi.nlm.nih.gov/pubmed/?term=Gray+1982+503] Note that in figure 3, the article describes a Gln (Q) in position 160.

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Reviews for Recombinant Human IFN-gamma Protein

Average Rating: 4.6 (Based on 22 Reviews)

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(Video) Webinar By Dr. Ashish on Process development of recombinant human interferon gamma using P. pastoris

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Recombinant Human IFN-gamma Protein

By Anonymous on 06/24/2022

Application: Flow Cytometry

Black -> HLA-E surface expression in T cells
Grey -> HLA-E surface expression in T cells upon IFNg stimulation (12h)

Recombinant Human IFN-gamma Protein

By Anonymous on 06/24/2022

Application: In vitro bioactivity in cell culture
Reason for Rating: Comparing gene expression responses by qPCR after IFNg treatment in immune cell subsets

More than a hundred-fold increase for a given target gene in immune cells stimulated with IFNg for 6h compared to controls (for basal level).

Recombinant Human IFN-gamma Protein

By Anonymous on 06/24/2022

Reason for Rating: Used for successfully stimulating human PBMCs.

Lane 1: PBMCs, unstimulated
Lane 2: PBMCs, stimulated with IFNg for 6h
Lane 3: PBMCs, stimulated with IFNg for 24h

Target: HLA detection

Recombinant Human IFN-gamma Protein

(Video) Interferons: INF-α, INF-β and INF-γ (FL-Immuno/07)

By Anonymous on 05/21/2021

Application: In vitro bioactivity in cell culture

Recombinant Human IFN-gamma Protein

By Marija Pieper on 03/11/2021

Application: Apoptosis assay

Recombinant Human IFN-gamma Protein

By Anonymous on 12/18/2020

Recombinant Human IFN-gamma Protein

By Anonymous on 11/20/2020

Application: Stem/Immune cell maintenance or differentiation
Reason for Rating: Gave consistent M1-like macrophage differentiation of human PBMCs

Recombinant Human IFN-gamma Protein

By Anonymous on 10/16/2020

Application: In vitro bioactivity in cell culture
Reason for Rating: Easy to solubilize

Used the to stimulate ex vivo skin culture

Recombinant Human IFN-gamma Protein

By Anonymous on 10/09/2020

Application: In vitro bioactivity in cell culture

HCT-116 cells were treated with hr-IFN-gamma for 24 hours and intracellular IDO-1 expression was measured.

Recombinant Human IFN-gamma Protein

By Anonymous on 06/08/2020

Application: In vitro bioactivity in cell culture

Recombinant Human IFN-gamma Protein

By Anonymous on 07/26/2019

Application: Cell Proliferation

By Anonymous on 07/25/2019

(Video) Interferon; Types, its receptor and mechanism of action

Application: In vitro bioactivity in cell culture
Reason for Rating: Consistent results when used for in vitro cell culture

CD38 transcript levels measured by qPCR after 24 hours treatment with 10 ng/mL IFN-g in MV-4-11 cells

Recombinant Human IFN-gamma Protein

By Anonymous on 06/09/2019

Application: In vitro bioactivity in cell culture
Reason for Rating: The expression of HLA-DR increased with MDAMB231 cells incubated with 1000 IU mL–1 IFN-gamma for 72 h

By Mehdi Alavi on 03/19/2019

Application: Enzymatic activity in vitro

Recombinant Human IFN-gamma Protein

By Anonymous on 11/16/2018

Application: Cell Proliferation
Reason for Rating: HT-29 Cell lines were treated with 100 U/ml IFN gamma, after which HLA-DR expression was examined by flow cytometer

By Anonymous on 10/08/2018

Application: In vitro bioactivity in cell culture

By Pia Muyot on 04/26/2017

Recombinant Human IFN-gamma Protein

By Anonymous on 07/21/2016

Application: In vitro bioactivity in cell culture
Reason for Rating: The IFN-g was excellent in stimulating whole blood.

IFN-g was used to stimulate whole blood. 100ng/mL final concentration was used in 1mL whole blood for 15 minutes at 37C. The cells were fixed and stored in -80C until further analysis on CyTOF

Recombinant Human IFN-gamma Protein

By Adam Guess on 07/13/2016

Application: In vivo study

IFN-gamma treatment (solid line) induces HLA-DR expression in cultured MSC as compared to control cells (filled histogram).

Recombinant Human IFN-gamma Protein

By shu wang on 05/31/2016

Application: In vitro bioactivity in cell culture

FAQs

What is recombinant human interferon? ›

Recombinant human interferon gamma (with HSA)

Interferon-gamma (IFN-γ), a Type II interferon, is secreted from CD4+ T-helper 1 (Th1) cells and activated natural killer (NK) cells. It plays a role in activating lymphocytes to enhance anti-microbial and anti-tumor effects.

Is IFN-gamma A protein? ›

IFN gamma is a dimeric protein consisting of two 146 amino acid subunits. IFN gamma is a glycoprotein that exists, functionally, as a homodimer of approximately 45 kDa. On SDS-PAGE, IFN gamma appears as a combination of 25, 20 and minor 15.5 kDa bands as a result of differential glycosylation.

What is IFN-gamma used for? ›

Interferon gamma-1b injection is used to reduce the frequency and severity of serious infections in people with chronic granulomatous disease (an inherited immune system disease). It is also used to slow down worsening of their condition in people with severe, malignant osteopetrosis (an inherited bone disease).

What is human interferon gamma? ›

Human interferon gamma is a cytokine belonging to a diverse group of interferons which have a crucial immunological function against mycobacteria and a wide variety of viral infections.

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